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KMID : 0356420010190030163
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Journal of Korean Andrology
2001 Volume.19 No. 3 p.163 ~ p.171
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Evidence for Peripheral Effect of Serotonin on Ejaculatory Response
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Paick Jae-Seung
Park Dal-Woo
Son Hwan-Cheol
Lee Sung-Ho
Kim Soo-Woong
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Abstract
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Purpose: Although serotonin (5-HT) has been implicated in central control of sexual desire, erection and ejaculation, its peripheral effect has been poorly studied. Here, we investigated the peripheral effects of 5-HT on ejaculation using in vivo and in vitro experiments.
Materials and Methods: Male Sprague-Dawley rats (weighed 250∼300 g) were injected intravenously with serotonergic agents (serotonin, fluoxetine, clomipramine) at various concentrations 20 min before electrical nerve stimulation of the hypogastric nerve. Intraluminal pressure of seminal vesicle and vas deferens was measured on each side in the same animal and compared. Total RNA was isolated from brain, seminal vesicle and vas deferens. Expressions of mRNAs for 5-HT1A, 5-HT1B and 5-HT2C receptors, which have been suggested for ejaculatory responses, were examined using semi-quantitative reverse-transcriptase polymerase chain reaction.
Results: All serotonergic agents caused dose-dependent inhibition of elevation in intraluminal pressure of the seminal vesicle. Vasal pressure responses were effectively inhibited by clomipramine and serotonin (clomipramine>serotonin), whereas no effect was seen by fluoxetine. There was no significant difference in relative expression levels of 5-HT1A receptor mRNAs between the seminal vesicle and vas deferens. However, the expression levels of 5-HT1B and 5-HT2C receptors mRNAs were lower in the vas deferens when comparing to those in the seminal vesicle.
Conclusions: These results of in vivo and in vitro experiments provide evidence for the peripheral role of 5-HT in regulating ejaculatory response of male rats. Regional differences in distributions of 5-HT receptor subtypes between seminal vesicle and vas may contribute to the different response to serotonergic agents in these organs.
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KEYWORD
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Ejaculation, Serotonin, Peripheral,
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